BIOSYNTHESIS OF A LYSINE-RICH HISTONE IN ISOLATED CALF THYMUS NUCLEI

Biosynthesis of a Lysine-rich Histone in Isolated Calf Thymus Nuclei.

Amino acid sequence of the COOH-terminal portion of the arginine-lysine-rich histone of calf thymus.

1. Highly purified arginineand lysine-rich histone was maleylated and then hydrolyzed with trypsin. A large amount of precipitate approximating 35% of the histone appeared during the demaleylation procedure. The precipitate was separated into four fractions on a Sephadex G-50 column and one fraction, tryptic peptide II from the maleylated histone, was found to be the COOH-terminal portion of th...

Chromatographic fractionation of calf thymus histone.

Evidence has recently accumulated that histone preparations, particularly those derived from the nuclei of calf thymus, may contain a mixture of different basic proteins. Variations in the amino acid composition of histone fractions extracted and precipitated by different means have been demonstrated by Stedman and Stedman (2), Daly, Mirsky, and Ris (3), Eadie and Leaf (4), Hamer (5), Davison a...

Studies on the Permeability of Calf Thymus Nuclei Isolated in Sucrose

A study of the permeability of calf thymus nuclei isolated in sucrose was carried out with sucrose-14C, glycerol-14C, and carboxydextran-14C (molecular weight, 60,000-90,000). The results indicate that the nuclei are very permeable to both sucrose and glycerol but they exclude the carboxydextran. Results obtained with other low molecular weight non-electrolytes (malonamide-14C, erythritol-14C, ...

Immunochemical specificity in lysine-rich histone subfractions.

Antibodies to purified calf thymus Fl, and to chromatographically purified subfractions of calf thymus Fl histones were induced by immunization of rabbits with histone-RNA complexes. Complement fixation studies with these antibodies demonstrated structural differences between the subfractions in one organ and between those of different organs and species. The anti-F1 sera showed varying reactiv...